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LINUX Cluster Project

Berechnung bioverfahrenstechnischer Probleme


Institution

  • Name: Lehrstuhl für Bioverfahrenstechnik
  • Address: Boltzmannstraße 15, 85748 Garching
  • Project Proposal Date: 2016-06-30 14:30:46

Abstract:

1. Project Preparative chromatography is a well-established technique for the purification of macromolecular bioproducts, especially therapeutic proteins. Under technical operating conditions, a steady decrease of the purification efficiency of chromatography columns due to medium wall detachment, formation of flow channels, or irreversible bed compression may occur. These effects can be caused by the complex compressibility behavior of the chromatography medium under high cyclic hydrodynamic stress. Therefore, in order to maintain constant purification efficiency and product quality, the transient hydrodynamic behavior of the column medium needs to be taken under consideration. The research project aims at the rigorous analysis and modeling of the transient hydrodynamic behavior of chromatography columns considering the mechanical and thermodynamic properties of different chromatography media. Mathematical modeling of the transient hydrodynamic behavior will be performed in coupled CFD-DEM simulations. Basic elements of the mathematical modeling is the coupling of the fluid and the solid particles and the calculation of particle trajectories and positions. The developed model will be validated using the obtained experimental data. 2. Project During the last decades diminishing reserves of crude oil as well as the increasing accumulation of greenhouse gasses in the atmosphere have lead to a reconsideration of utilisation of fossil ressources as fuels. Thus, to maintain the current aerospace growth rate and since european regulation requires a decrease of greenhouse gas emissions, new feasible solutions for the production of renewable fuels have to be developed. One promising approach is the use of microalgae that convert carbon dioxide and sunlight photosynthetically to energy-rich lipids. This requires large-area photobioreactors to ensure the necessary supply with sunlight. However, due to the very low added value, only open photobioreactors with minimal investment and operating costs can be considered. For the development of new reactors and the operation with new microalgae, a process simulation of algal growth and product formation in different photobioreactors is of great significance to reduce the experimental input. Therefore the objective of this research project is to create a computational fluid dynamics (CFD) model, which allows the modelling of the complex interaction between reactor geometry, microalgae kinetics and reaction conditions. Exploiting varying process conditions, a process simulation of open photobioreactors and thus a comparison of different reactor concepts will be performed. 3. Project Chiral compounds are important building blocks for the synthesis of bioactive compounds, e.g., pharmaceuticals, food and feed additives, and agrochemicals. The asymmetric reduction of alkenes represents an interesting approach for the production of these chiral molecules. Although cis-hydrogenations are well-established in industry, trans-hydrogenations remain challenging for chemical catalysis. Here biocatalysts can represent an ecologically and economically beneficial alternative. Due to their broad substrate spectrum, high catalytic activity and stereoselectivity, ene-reductases from cyanobacteria are promising biocatalysts for the trans-specific reduction of alkenes. However, cyanobacterial ene-reductases prefer NADPH to the more stable and cheaper alternative NADH, which hinders their possible industrial application. The objective of this research is the rational optimisation of the cofactor specificity of cyanobacterial ene-reductases using different protein-engineering methods. These optimised biocatalysts will be characterised for their applicability for the production of (2R,5R)-dihydrocarvone in a batch process using isolated enzymes or whole-cells. In addition, two-phase systems will be investigated and compared to the monophasic reaction setups. The process with the highest space-time yield and highest diastereomeric excess of the product will be scaled up to the liter-scale. These studies are accomplished in the context of the project "Resource-efficient production of fine chemicals". The project is part of the network "Resource-efficient biotechnology in Bavaria – BayBiotech", which is financed by the Bavarian State Ministry of the Environment and Consumer Protection (www.baybiotech.de).